Abolition of the protected phenotype by administration of diphtheria toxin to clodronate-treated mice suggested that the protective effect of clodronate resulted from the presence of a cytoprotective intrarenal population of mononuclear phagocytes sensitive to diphtheria toxin–mediated ablation. Nihon Saikingaku Zasshi. At a certain intracellular concentration, clodronate induces apoptosis of the macrophage. COVID-19 is an emerging, rapidly evolving situation. Further details on each reagent is available by clicking on “More Info” below its image. Leong KG, Ozols E, Kanellis J, Nikolic-Paterson DJ, Ma FY. Depletion of cells of monocyte lineage prevents loss of renal microvasculature in murine kidney transplantation. Liposomal clodronate was prepared as described previously. Macrophage depletion can be achieved with the systemic injection of liposomes containing clodronate.6 Clodronate be-longs to the family of bisphosphonates (BPs), bone-seeking agents that are potent inhibitors of osteoclasts. However, we have observed in numerous rodent studies that liposomes which contain PBS instead of clodronate solution do not elicit antitumor activity following i.v. The purpose of this study was to develop a method for the depletion of macrophages from the peritoneal cavity and the omentum of the rat. If clodronate liposomes are being used for complete systemic depletion of macrophages, the rate of repopulation of the phagocytes within the various organs and blood must be taken into account, especially considering that monocyte counts begin to increase between 24-30 hours in mice while liver and splenic macrophages may remain depleted for up to a week or more. Am J Physiol Renal Physiol. Qi F, Adair A, Ferenbach D, Vass DG, Mylonas KJ, Kipari T, Clay M, Kluth DC, Hughes J, Marson LP. Prolonged depletion of Gr1−monocytes was observed. Macrophage/monocyte depletion by clodronate, but not diphtheria toxin, improves renal ischemia/reperfusion injury in mice. To evaluate mononuclear phagocytic cell effects in our rUUO model of CKD, clodronate or PBS control liposomes were administrated to mice intravenously. Interestingly, this treatment caused a delayed disappearance (7-21 days post injection) of macrophages and … injection of clodronate liposomes, and the population is not restored for 2 wk thereafter . 1993 Sep;48(5):685-92. doi: 10.3412/jsb.48.685. Van Rooijen, N., & Hendrikx, E. (2010). Epub 2020 Aug 24. Liposomes Macrophages Clodronate Depletion of macrophages Kupffer cells Spleen Liver Lung Lymph nodes This is a preview of subscription content, log in to check access. Flow cytometric analysis revealed that clodronate-liposome pretreatment impaired myeloma plasma cell homing and retention within the bone marrow 24 hours postmyeloma plasma cell inoculation. This pharmacological treatment has been demonstrated to induce specific apoptotic death in monocytes and phagocytic macrophages. C57BL/6 mice require a higher dose of cisplatin to induce renal fibrosis and CCL2 correlates with cisplatin-induced kidney injury. We use cookies to help provide and enhance our service and tailor content and ads. In the current studies, the liposomal clodronate … OBJECTIVE: To assess whether intraarticular (IA) administration of clodronate liposomes results in local macrophage depletion in patients with rheumatoid arthritis (RA). Published by Elsevier Inc. All rights reserved. In contrast, DT treatment did not ameliorate the acute tubular necrosis score (Figure A single IA administration of clodronate liposomes leads to macrophage depletion and decreased expression of adhesion molecules in the synovial lining in patients with longstanding RA. National Center for Biotechnology Information, Unable to load your collection due to an error, Unable to load your delegates due to an error. This pharmacological treatment has been demonstrated to induce specific apoptotic death in monocytes and phagocytic macrophages. The procedure is well tolerated, and its therapeutic potential is currently under investigation. Depletion of macrophages and dendritic cells in ischemic acute kidney injury. Macrophage depletion by clodronate liposome attenuates muscle injury and inflammation following exhaustive exercise Noriaki Kawanishia,b, Tsubasa Mizokamic, Hiroyuki Niiharac, Koichi Yadac, Katsuhiko Suzukid,n a Institute for Nanoscience & Nanotechnology, Waseda University, Tokyo, Japan b Research Fellow of the Japan Society for the Promotion of Sciences, Tokyo, Japan administration of 2.5 mg/kg liposome-encapsulated clodronate at a volume of 0.5 ml/kg over a 60-minute period via an indwelling venous catheter, as previously described. 2021 Jan 18. doi: 10.1038/s41569-020-00489-x. Get the latest public health information from CDC: https://www.coronavirus.gov, Get the latest research information from NIH: https://www.nih.gov/coronavirus, Find NCBI SARS-CoV-2 literature, sequence, and clinical content: https://www.ncbi.nlm.nih.gov/sars-cov-2/. Macrophage depletion by clodronate liposomes also reduced intimal hyperplasia, the. To help resolve this, we used mice transgenic for the human diphtheria toxin receptor under the control of the CD11b promoter and treated them with diphtheria toxin, or liposomal clodronate, or both to deplete monocyte/mononuclear phagocytes prior to renal ischemia/reperfusion injury. Clophosome ®, neutral clodronate liposomes, offers highly efficient in vivo macrophage depletion. Mice were injected with clodronate liposomes or PBS liposomes 2 days (0.2 ml iv of liposomes through the tail vein) and 2 h (0.1 ml ip of liposomes) before muscle injury. Mononuclear phagocyte depletion strategies in models of acute kidney disease: what are they trying to tell us? Although either system effectively depleted circulating monocytes and resident mononuclear phagocytes, depletion was most marked in diphtheria toxin-treated mice. Monocyte depletion diminishes renal injury and neutrophil responses.